中心粒細(xì)胞會(huì)被巨噬細(xì)胞清除劑Clodronate Liposomes清除嗎？

巨噬細(xì)胞清除劑Clodronate Liposomes氯膦酸鹽脂質(zhì)體廣泛用于體內(nèi)和體外清除單核巨噬細(xì)胞，我們?cè)谑褂煤商mLiposoma巨噬細(xì)胞清除劑時(shí)，盡管該產(chǎn)品頻頻見刊于Cell，Nature和Science。但是巨噬細(xì)胞清除劑會(huì)不會(huì)清除中心粒細(xì)胞？很多研究者有這個(gè)擔(dān)心。一方面我們可以在自己的模型里面驗(yàn)證，另一方面，我們也可以參考文獻(xiàn)。比如如下這篇JCI的老牌雜志文獻(xiàn)，炎性疼痛模型。作者的數(shù)據(jù)證明：巨噬細(xì)胞清除劑Clodronate Liposomes氯膦酸鹽脂質(zhì)體（Liposoma）不會(huì)清除中心粒細(xì)胞。

論文信息：

論文題目： GPR37 regulates macrophage phagocytosis and resolution of inflammatory pain

期刊名稱：J Clin Invest.

時(shí)間期卷：2018;128(8):3568–3582.

在線時(shí)間：2018年7月16日

DOI：doi.org/10.1172/JCI99888.

Depletion of hindpaw skin macrophages (sMΦ) by systemic injection of clodronate-liposome.  

(A) Flow cytometry images showing a reduction of F4/80+ CD11b+ sMΦ in inflamed hind paw skins after intraperitoneal

injections (15 µl/g, 2 and 48 h prior to the zymosan injection) of clodronate-liposome. Right panel, quantification of the

data.

 (B) Flow cytometry showing no reduction of Gr1+ CD11b+ neutrophils in inflamed hind paw skins after

clodronate-liposome treatment. Right panel, quantification of the data.   n = 3 mice /group, *P<0.05,

clodronate vs. control, #P<0.05, compared with contralateral, ns, not significant. All the data are mean ± SEM.